ABSTRACT
<p><b>AIM</b>To evaluate the effect of single or dual field irradiation (IR) with the same dose on damage to miniature pig parotid glands.</p><p><b>METHODOLOGY</b>Sixteen miniature pigs were divided into two IR groups (n=6) and a control group (n=4). The irradiation groups were subjected to 20 Gy X-radiation to one parotid gland using single-field or dual-field modality by linear accelerator. The dose-volume distributions between two IR groups were compared. Saliva from parotid glands and blood were collected at 0, 4, 8 and 16 weeks after irradiation. Parotid glands were removed at 16 weeks to evaluate tissue morphology.</p><p><b>RESULTS</b>The irradiation dose volume distributions were significantly different between single and dual field irradiation groups (t=4.177, P=0.002), although dose volume histogramin (DVH) indicated the equal maximal dose in parotid glands. Saliva flow rates from IR side decreased dramatically at all time points in IR groups, especially in dual field irradiation group. The radiation caused changes of white blood cell count in blood, lactate dehydrogenase and amylase in serum, calcium, potassium and amylase in saliva. Morphologically, more severe radiation damage was found in irradiated parotid glands from dual field irradiation group than that from single field irradiation group.</p><p><b>CONCLUSION</b>Data from this large animal model demonstrated that the radiation damage from the dual field irradiation was more severe than that of the single field irradiation at the same dose, suggesting that dose-volume distribution is an important factor in evaluation of the radiobiology of parotid glands.</p>
Subject(s)
Animals , Male , Amylases , Blood , Radiation Effects , Blood Platelets , Radiation Effects , Calcium , Radiation Effects , Erythrocyte Count , Erythrocytes , Radiation Effects , L-Lactate Dehydrogenase , Blood , Radiation Effects , Leukocyte Count , Leukocytes , Radiation Effects , Models, Animal , Organ Size , Radiation Effects , Parotid Gland , Pathology , Radiation Effects , Potassium , Radiation Effects , Radiation Dosage , Random Allocation , Saliva , Chemistry , Radiation Effects , Secretory Rate , Radiation Effects , Swine , Swine, Miniature , Time FactorsABSTRACT
<p><b>OBJECTIVE</b>To investigate the targeting expression of TRAIL gene driven by human telomerase reverse transcriptase (hTERT) promoter in SACC-83 cell with telomerase activity.</p><p><b>METHODS</b>Adenovirus vector AdTERT-TRAIL was constructed by homologus recombination. After transfecting AdTERT-TRAIL into SACC-83 cell and HEL cell, its effect on these cells in vitro was investigated using RT-PCR technique, MTT method and flow cytometry.</p><p><b>RESULTS</b>After transfection of AdTERT-TRAIL, expression of extrinsic TRAIL gene driven was detected in SACC-83, the proliferation of SACC-83 cell showed significant inhibitory effect (the relative cell viability was 49.70%) and its apoptotic rate was promoted (30.49%), whereas no TRAIL gene was detected in HEL cell, also no inhibitory effect was observed in HEL cell and its apoptotic rate showed little change.</p><p><b>CONCLUSION</b>Adenovirus vector AdTERT-TRAIL was successfully constructed, which can be used to induce expression of TRAIL gene in SACC-83 cell with targeting effect.</p>